Journal: Cell Reports Methods

Article Title: Protein fluorescent labeling in live yeast cells using scFv-based probes

doi: 10.1016/j.crmeth.2022.100357

Figure Lengend Snippet:

Article Snippet: ALLInTM RED Taq Mastermix 2x , HighQu , Cat# PCM0201c1.

Techniques: Recombinant, Staining, Saline, Plasmid Preparation, Microscopy, Western Blot, Amplification, Expressing, Labeling, Software, Membrane, Stripping Membranes, Transferring, Imaging, Spectrophotometry

Journal: bioRxiv

Article Title: Link between aging and atheroprotection in Mif -deficient atherosclerotic mice

doi: 10.1101/2021.12.14.471281

Figure Lengend Snippet: A-B ) mRNA expression of MIF was assessed by RT-qPCR in spleen ( A ) and liver ( B ) of Apoe −/– mice after 24 and 42 weeks of HFD. Values were normalized to the values of the 30-week-old Apoe −/– controls that received HFD for 24 weeks (n=5-7 mice; results are presented as means ± SD). C ) MIF plasma levels as measured by ELISA in Apoe −/– mice after 24 and 42 weeks of HFD. Values were normalized to the Apoe −/– controls that received HFD for 24 weeks (n=8-9 mice per group; results are presented as means ± SD). Statistics: Mann-Whitney test (no significant differences observed; each data points represents one mouse).

Article Snippet: RT-qPCR was performed using ORA TM SEE qPCR Green ROX H Mix (HighQu, Kraichtal, Germany) and specific mouse primer pairs (Eurofins, Ebersberg, Germany).

Techniques: Expressing, Quantitative RT-PCR, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

Journal: bioRxiv

Article Title: Link between aging and atheroprotection in Mif -deficient atherosclerotic mice

doi: 10.1101/2021.12.14.471281

Figure Lengend Snippet: mRNA expression of FoxP3 was assessed by RT-qPCR in spleen of Apoe −/– Mif −/– (blue) after 24 and 42 weeks of HFD and compared to the WT controls (grey). Values were normalized to the values of the 30-week-old Apoe −/– controls that received HFD for 24 weeks (n=6-10 mice per group; results are presented as means ± SD). Statistics: two- way ANOVA (each data point represents one mouse).

Article Snippet: RT-qPCR was performed using ORA TM SEE qPCR Green ROX H Mix (HighQu, Kraichtal, Germany) and specific mouse primer pairs (Eurofins, Ebersberg, Germany).

Techniques: Expressing, Quantitative RT-PCR